Stool White Blood Cells (WBC) Test

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1. Objective:
The objective of this test was to detect the presence of white blood cells (WBCs) in the stool sample, which indicated inflammation or infection in the gastrointestinal tract.

2. Principle:
The test was based on the principle that WBCs migrate to the site of infection or inflammation in the intestine. Their presence in stool suggested an invasive bacterial infection (e.g., Shigella, Salmonella, Campylobacter) or inflammatory bowel disease.

3. Materials Used:
Fresh stool sample
Glass slides and coverslips
Methylene blue or Gram stain
Applicator sticks
Microscope
Dropper and saline

4. Procedure (Microscopic):
1. A small amount of stool was placed on a glass slide.
2. A drop of saline or methylene blue was added and mixed gently.
3. A coverslip was placed on the mixture.
4. The slide was examined under a microscope under low and high power.
5. The presence and number of WBCs were recorded.

5. Result:
Positive: WBCs were observed, indicating inflammation or infection.
Negative: No WBCs were seen, suggesting the absence of invasive infection.

6. Uses:
To differentiate between invasive and non-invasive diarrhea.
To evaluate suspected cases of bacterial dysentery or IBD.
To aid in diagnosing conditions like ulcerative colitis or Crohn’s disease.

7. Consultation:
The result was interpreted alongside clinical symptoms and other stool test results. A positive finding required further microbiological tests or colonoscopic evaluation for a definitive diagnosis and treatment planning.

[C] MediLab BD

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