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1. Why is EDTA used in CBC tests?
EDTA prevents blood from clotting by binding calcium, ensuring accurate cell counts.
2. Why is fasting required before a lipid profile test?
Food intake can temporarily raise lipid levels, so fasting ensures accurate results.
3. Why is Ziehl-Neelsen stain used for Mycobacterium?
Its waxy cell wall retains carbol fuchsin even after acid-alcohol wash.
4. Why is centrifugation used in clinical labs?
To separate blood into plasma, serum, and cells based on density.
5. Why is heparin used in arterial blood gas (ABG) tests?
It prevents clotting without affecting pH or gas composition.
6. Why is urine collected midstream?
To avoid contamination from external genitalia and ensure accurate analysis.
7. Why should hemolysis be avoided in blood samples?
Hemolysis alters values like potassium, LDH, and can ruin test results.
8. Why are blood samples stored cold?
To prevent breakdown of chemicals and preserve sample integrity.
9. Why is sodium fluoride used for glucose estimation?
It blocks glycolysis, preserving true glucose levels in the sample.
10. Why is 70% alcohol used for disinfection?
It's most effective in penetrating microbial walls and denaturing proteins.
11. Why is cross-matching done before transfusion?
To prevent serious reactions from blood group incompatibility.
12. Why is daily quality control important in labs?
To maintain accuracy, consistency, and credibility of lab results.
13. Why is Gram staining important?
It classifies bacteria for diagnosis and guides antibiotic treatment.
14. Why are control samples used during tests?
To verify that the test is functioning correctly and reliably.
15. Why is bilirubin checked in liver tests?
To assess liver function and detect bile duct obstruction or hemolysis.
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