Posts

Eagles Eye Update

Gram stain Procedure and Organisms

Image
The Gram stain is a fundamental laboratory technique used to differentiate bacterial species based on the chemical and physical properties of their cell walls. Here's a breakdown of the procedure and how it relates to different organisms: GRAM STAIN PROCEDURE : The Gram stain procedure involves a series of steps:  * Crystal Violet (Primary Stain) :    * This stains all bacterial cells purple.  * Gram's Iodine (Mordant) :    * This forms a complex with the crystal violet, trapping it within the cell wall.  * Decolorization (Alcohol or Acetone):    * This step is crucial.    * Gram-positive bacteria, with their thick peptidoglycan layer, retain the crystal violet-iodine complex and remain purple.    * Gram-negative bacteria, with their thinner peptidoglycan layer and outer membrane, lose the crystal violet-iodine complex and become colorless.  * Safranin (Counterstain):    * This stains the decolor...

Gram Positive and Culture Media

Image
When working with Gram-positive bacteria in a laboratory setting, culture media plays a crucial role in their identification and study. Here's how Gram-positive bacteria interact with different types of culture media: General Principles: Nutrient Requirements :    * Like all bacteria, Gram-positive organisms need specific nutrients to grow. Culture media provides these essential elements, including carbon sources, nitrogen sources, vitamins, and minerals. Selective vs. Differential Media:    * Culture media can be either selective, differential, or both.    * Selective media inhibits the growth of certain bacteria while allowing others to grow. This is useful for isolating specific Gram-positive species from a mixed population.    * Differential media contains substances that allow different bacteria to be distinguished based on their metabolic reactions. This helps in identifying specific species. Examples of Culture Media and Gram-Po...

Compare and Contrast between Peptidoglycan layer and Techoic acid of Bacteria

Image
To understand the differences and similarities between peptidoglycan and teichoic acids, it's essential to consider their roles within the Gram-positive bacterial cell wall. Here's a comparative breakdown: PEPTIDOGLYCAN LAYER: Structure :    * It's a polymer composed of sugars and amino acids that form a mesh-like layer.    * It creates a rigid, thick structure surrounding the cell. Function :    * Provides structural integrity and rigidity to the cell wall.    * Protects the cell from osmotic pressure.    * It is the layer that retains the crystal violet stain in gram positive bacteria.   Location :    * Forms the primary component of the Gram-positive cell wall, located outside the plasma membrane. TECHOIC ACIDS :   Structure :    * Polymers of glycerol phosphate or ribitol phosphate.    * Can be divided into Wall teichoic acids, and lipoteichoic acids.   Function :    ...

Gram Positive Cell Walls

Image
The Gram-positive cell wall is a key feature that distinguishes this group of bacteria. Here's a breakdown of its structure and characteristics: Key Components : Peptidoglycan :    * This is the dominant component, forming a thick, multilayered structure. It provides the cell with structural integrity and protection against osmotic pressure.    * It's a polymer of sugars and amino acids that are cross-linked, creating a strong, mesh-like network. Teichoic Acids:    * These are embedded within the peptidoglycan layer.    * They are polymers of glycerol phosphate or ribitol phosphate.   There are two main types:       Wall teichoic acids : These are covalently linked to the peptidoglycan.       Lipoteichoic acids : These are linked to the underlying plasma membrane.    * Teichoic acids contribute to the cell's negative charge, regulate ion movement, and play a role in cell growth and adhesion....

UK to deport many Nigerian nurses over examination fraud

Image
The United Kingdom’s Home Office has asked some Nigerian nurses being probed for alleged examination fraud to leave the country. According to Nursing Times, the affected nurses are still awaiting the outcomes of their appeals. However, they have received official letters from the Home Office instructing them to leave the UK starting next week. The deportation order follows an ongoing probe by the UK’s Nursing and Midwifery Council (NMC) into suspected malpractice at Yunnik Technologies Test Centre in Ibadan, Nigeria. Campaigners and advocates have called on the regulator to expedite hearings to prevent the nurses from being repatriated before their appeals are fully considered. Peters Omoragbon, executive president of Nurses Across The Borders and president of the Diaspora Nurses Association of Nigeria, accused the NMC of “deliberately delaying” appeal hearings so that nurses could be served with notice from the Home Office. He told Nursing Times: “That saves them the troub...

Uphold Ethical Standards And Reduce Misconduct To Foster Public Trust - MLSCN Registrar/CEO tells Medical Laboratory Science researchers

Image
Professor Tosan Erhabor has urged Medical Laboratory Science researchers to uphold ethical standards and reduce misconduct to foster public trust. He made this call through his representative, Dr. Akinwale Akinlabi, Director Health Laboratory Planning, Research & Statistics, during a training session organized by the Medical Laboratory Science Council of Nigeria (MLSCN) Health Research Ethics Committee (HREC) on Health Policy and Planning (HPP). The three-day training, which focused on reviewing research protocols and providing constructive feedback, commenced today in Abuja Speaking at the occasion, the Chairman of the MLSCN Ethics Review Committee (ERC), Professor Kolawole Solomon Oyedeji, emphasized the need for strict compliance with ethical standards to enhance the quality of research. He highlighted that adherence ethical guidelines ensures credible and reliable research outcomes, ultimately benefiting society. The training brought together key stakeho...

Former Ogun AMELTAN Scribe pen down from service of Sacred Heart Hospital after 19 years

Image
The Former General Secretary of Association Of Medical Laboratory Technicians and Assistants of Nigeria (AMELTAN), Ogun state chapter, Com Osho Azeez Olatunde , pen down his service withdrawal letter from Sacred Heart Hospital Lantoro, after 19 years of service at the facility. The letter reads: 28th February, 2025 Dearest Colleagues, As I pen this letter, I'm filled with a mix of emotions - sadness to leave behind the familiar, yet excitement for the new chapter ahead. Today marks my last day at Sacred Heart Hospital, Lantoro, Abeokuta, Ogun State, after an incredible 19-year journey. I'm grateful for the opportunity to bid you all farewell and to reflect on the memories, lessons, and experiences gained during my time here. Each of you has contributed to my growth, and I'll always cherish the relationships built and the lives touched. Nineteen years ago, I embarked on this journey with passion, dedication, and a commitment to delivering exceptional healthcare s...