Gram stain Procedure and Organisms
The Gram stain is a fundamental laboratory technique used to differentiate bacterial species based on the chemical and physical properties of their cell walls. Here's a breakdown of the procedure and how it relates to different organisms: GRAM STAIN PROCEDURE : The Gram stain procedure involves a series of steps: * Crystal Violet (Primary Stain) : * This stains all bacterial cells purple. * Gram's Iodine (Mordant) : * This forms a complex with the crystal violet, trapping it within the cell wall. * Decolorization (Alcohol or Acetone): * This step is crucial. * Gram-positive bacteria, with their thick peptidoglycan layer, retain the crystal violet-iodine complex and remain purple. * Gram-negative bacteria, with their thinner peptidoglycan layer and outer membrane, lose the crystal violet-iodine complex and become colorless. * Safranin (Counterstain): * This stains the decolor...