Stool Culture Test

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1. Objective:
The objective of the stool culture test was to isolate and identify pathogenic bacteria in a patient's fecal sample, particularly those causing gastrointestinal infections.

2. Principle:
The test was based on the cultivation of stool specimens on selective and differential media to promote the growth of enteric pathogens while inhibiting normal flora. Biochemical and serological methods were then used to identify specific organisms.

3. Materials:
• Fresh stool sample
• Sterile stool collection container
• Inoculating loop
• Culture media: MacConkey agar, XLD agar, SS agar, Selenite F broth
• Incubator (35–37°C)
• Biochemical test kits (TSI, SIM, Citrate, Urease, etc.)
• Gram staining reagents
• PPE (gloves, mask, lab coat)

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4. Procedure (Microbiological):
1. The stool sample was collected in a sterile container and transported promptly to the lab.
2. Using an inoculating loop, a portion of the stool was streaked onto MacConkey, XLD, and SS agar plates.
3. The sample was also inoculated into Selenite F broth for enrichment.
4. All media were incubated at 37°C for 18–24 hours.
5. After incubation, plates were examined for colony morphology.
6. Suspected colonies were further identified using biochemical tests and Gram staining.
7. If required, serological tests were performed for confirmation (e.g., Salmonella and Shigella serotyping).

5. Result:
• Normal: No pathogenic organisms isolated; only normal gut flora observed.
• Abnormal: Growth of enteric pathogens like Salmonella spp., Shigella spp., E. coli (EHEC, ETEC), Campylobacter spp., or Vibrio cholerae.

6. Uses:
• Diagnosed causes of diarrhea, dysentery, or food poisoning
• Identified antibiotic-resistant enteric bacteria
• Traced outbreaks of enteric infections
• Assisted in public health surveillance

7. Consultation:
Positive stool culture results were interpreted by clinicians alongside clinical symptoms and history. Further antibiotic sensitivity testing guided therapy.


© Mr Adedeji Adeyanju via WhatsApp 

Comments

  1. It's a secretAugust 15, 2025 at 7:58 PM

    Wow. This is beautiful to read. Standard operating procedure explained for easier understanding.
    Thank you

    ReplyDelete

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